1X Modified Lysis Buffer
50mM HEPES 5.96 grams
150mM NaCl 4.38 grams
10% Glycerol 50mL
1% Triton X-100 5mL
1mM EDTA 0.186 grams
100mM NaF 2.10 grams
10mM NaPPi 2.23 grams
ddH2O Top up to 500mL
Store at 4degrees
Before use, add these to 1X Modified Lysis Buffer:
Additive Stock Solution Amount to add to 5mL Lysis buffer
PMSF (dissolve in DMSO) 100mM 50uL
Store at -20​
Na3VO4 200mM 25uL
Store at -20​
B-Glycerophosphate 500mM 100uL
Store at RT​
Sodium Deoxycholate 10% (w/v) in water 500uL [Final] in sol’n =1%
Store at RT. Tin Foil.​
Roche Complete Mini Tablets 1/2 Tablet
Store at 4 deg​
Cat#​ 11 836 153 001
Lot# 14015000