1X Western Blot Semi-Dry Transfer Buffer

0.48mM Tris                  11.62 grams
39mM Glycine               5.86 grams
0.037% (w/v) SDS          0.75 grams
20% Methanol               400mL
ddH2O                            Top up to 2L

Store at 4degres

*Use this when transferring onto a PVDF membrane

Mailing Address

Arthur and Sonia Labatt Brain Tumour Research Centre

The Hospital for Sick Children

Peter Gilgan Centre for Research and Learning

686 Bay Street, 17-9702
Toronto, ON  M5G 0A4