4X Loading Dye

3X Protein Denaturing Sample Loading Buffer (Loading Dye)
 

1.5M Tris (pH 6.8)                1.5mL​
20% SDS                                0.9 mL
Glycerol                                 4.5mL
B-mercaptoethanol             2.1mL
Bromophenol Blue              0.26mg


ddH2O Top up to 10mL


Make 1mL aliquots and store at -20