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4X Loading Dye
3X Protein Denaturing Sample Loading Buffer (Loading Dye)
1.5M Tris (pH 6.8) 1.5mL
20% SDS 0.9 mL
Glycerol 4.5mL
B-mercaptoethanol 2.1mL
Bromophenol Blue 0.26mg
ddH2O Top up to 10mL
Make 1mL aliquots and store at -20
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