4X Loading Dye

3X Protein Denaturing Sample Loading Buffer (Loading Dye)

1.5M Tris (pH 6.8)                1.5mL​
20% SDS                                0.9 mL
Glycerol                                 4.5mL
B-mercaptoethanol             2.1mL
Bromophenol Blue              0.26mg

ddH2O Top up to 10mL

Make 1mL aliquots and store at -20

Mailing Address

Arthur and Sonia Labatt Brain Tumour Research Centre

The Hospital for Sick Children

Peter Gilgan Centre for Research and Learning

686 Bay Street, 17-9702
Toronto, ON  M5G 0A4