How to Make SDS-PAGE Gels

Resolving Gels

7.5% SDS-PAGE Gel (1gel)

ddH2O                            mL

40% Polyacrylamide     mL

4X Lower Gel Buffer     2.5mL

10% Ammonium Persulfate  45uL

TEMED                                      15uL

10% SDS-PAGE Gel (1gel)

ddH2O                            5mL

40% Polyacrylamide     2.5mL

4X Lower Gel Buffer     2.5mL

10% Ammonium Persulfate  45uL

TEMED                                      15uL

12.5% SDS-PAGE Gel (1gel)

ddH2O                            mL

40% Polyacrylamide     mL

4X Lower Gel Buffer     2.5mL

10% Ammonium Persulfate  45uL

TEMED                                      15uL

15% SDS-PAGE Gel (1gel)

ddH2O                            3.75mL

40% Polyacrylamide     3.75mL

4X Lower Gel Buffer     2.5mL

10% Ammonium Persulfate  45uL

TEMED                                      15uL

Stacking Gels

SDS-PAGE Gel (enough for 4gels)

ddH2O                            5mL

40% Polyacrylamide     0.9mL

4X Lower Gel Buffer     2mL

10% Ammonium Persulfate  48uL

TEMED                                      20uL