top of page

How to Make SDS-PAGE Gels

Resolving Gels

7.5% SDS-PAGE Gel (1gel)

ddH2O                            mL

40% Polyacrylamide     mL

4X Lower Gel Buffer     2.5mL

​

10% Ammonium Persulfate  45uL

TEMED                                      15uL

10% SDS-PAGE Gel (1gel)

ddH2O                            5mL

40% Polyacrylamide     2.5mL

4X Lower Gel Buffer     2.5mL

​

10% Ammonium Persulfate  45uL

TEMED                                      15uL

12.5% SDS-PAGE Gel (1gel)

ddH2O                            mL

40% Polyacrylamide     mL

4X Lower Gel Buffer     2.5mL

​

10% Ammonium Persulfate  45uL

TEMED                                      15uL

15% SDS-PAGE Gel (1gel)

ddH2O                            3.75mL

40% Polyacrylamide     3.75mL

4X Lower Gel Buffer     2.5mL

​

​

10% Ammonium Persulfate  45uL

TEMED                                      15uL

Stacking Gels

SDS-PAGE Gel (enough for 4gels)

ddH2O                            5mL

40% Polyacrylamide     0.9mL

4X Lower Gel Buffer     2mL

​

10% Ammonium Persulfate  48uL

TEMED                                      20uL

BTRC logo.png
SickKids-Logo.gif

Mailing Address

​

Arthur and Sonia Labatt Brain Tumour Research Centre

The Hospital for Sick Children

Peter Gilgan Centre for Research and Learning

686 Bay Street, 17-9702
Toronto, ON  M5G 0A4

Canada

​

bottom of page