How to Make SDS-PAGE Gels
Resolving Gels
7.5% SDS-PAGE Gel (1gel)
ddH2O mL
40% Polyacrylamide mL
4X Lower Gel Buffer 2.5mL
10% Ammonium Persulfate 45uL
TEMED 15uL
10% SDS-PAGE Gel (1gel)
ddH2O 5mL
40% Polyacrylamide 2.5mL
4X Lower Gel Buffer 2.5mL
10% Ammonium Persulfate 45uL
TEMED 15uL
12.5% SDS-PAGE Gel (1gel)
ddH2O mL
40% Polyacrylamide mL
4X Lower Gel Buffer 2.5mL
10% Ammonium Persulfate 45uL
TEMED 15uL
15% SDS-PAGE Gel (1gel)
ddH2O 3.75mL
40% Polyacrylamide 3.75mL
4X Lower Gel Buffer 2.5mL
10% Ammonium Persulfate 45uL
TEMED 15uL
Stacking Gels
SDS-PAGE Gel (enough for 4gels)
ddH2O 5mL
40% Polyacrylamide 0.9mL
4X Lower Gel Buffer 2mL
10% Ammonium Persulfate 48uL
TEMED 20uL